Hi people!
Stella here.
i'm currently attached to Biochemistry Lab.
as it is an automated lab, we get to see different types of machines and so i'll be mostly talking about analyzers.
The first week of work, i was assigned to the Beckman Coulter DxC 800 Analyzer.
Its function is to run different types of tests such as liver function test, renal function test and cardiac markers.
DxC 800 makes use of three principles in determining the sample results.
They are Enzymatic Method, ISE(Ion Selective Electrodes) Method and Spectrophotometry methods.
The analyzer contains different types of reagents, serving different purposes of the biochemical analysis. It is divided into two parts. They are cartridge chemistry which contains reagents such as ALT, AST, CK and many more. Whereas the modular chemistry, it contains sodium, potassium, chloride, glucose and many more.
Cartridge chemistry makes use of spectrophotometry methods whereby a substance will be produced and is able to absorb light at certain wavelengths. This happens when a sample is mixed together with the appropriate reagents.
As for modular chemistry, the ISE will convert the activity of a specific ion which will be dissoved in a solution into an electricity potential. The electrode dat senses is usually made up of an ion-specific membrane, alongside with a refernce electrode.
It is important to top up the reagents at different intervals during the day to make sure that reagents do not run out and patient samples can assessed without any disturbances.
Each time the reagents need to be topped up, the system will prompt the person on duty.
I was given the chance to top up the reagents, as well as loading the patient samples manually into the analyzer.
I also learnt all about calibration and quality control which have to be done every day, preferably in the morning.
As the lab is mostly automated, the analyzer plays an important role in producing results for the patient samples so that diagnosis and treatment of the diseases can be confirmed.
:)
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11 comments:
Hello Stella.
Could you explain to me with a given example of the process of enzymatic method conducted within the DxC? Any markers or analytes would do? =D
Tng Wess Lee
0702570C
TG02
Grp 10
hi wess!
an example of a marker/analyte that uses enzymatic method is CK(Creatine Kinase).
In the reaction, CK will catalyze the substrate, for example creatinine phosphate substrate and there will be formation of ATP. in this case, the reaction can be measured and the amount of CK can be determined based on the products of the reaction.
I hope it answers your question.
:)
stella
hello~
im curious, do you have to load all the samples into the machines? or is certain samples, then the other specimens are automated loaded into the machine?
and also are there any tests are to be done manually in ur department?
yaNLing xD (grp 7)
hello yanling!
yes we need to load all the samples into the machines so that the required tests can be performed.
and there are certain samples that cannot be loaded automatically into the machines. samples such as urine in PS tubes and serum in microtubes cannot enter the LAS automatically so we have to load them manually into the different machines.
and yes there are tests which are done manually such as Urine Phase Contrast.
i hope i answer your questions!
:)
hello yanling!
yes we need to load all the samples into the machines so that the required tests can be performed.
and there are certain samples that cannot be loaded automatically into the machines. samples such as urine in PS tubes and serum in microtubes cannot enter the LAS automatically so we have to load them manually into the different machines.
and yes there are tests which are done manually such as Urine Phase Contrast.
i hope i answer your questions!
stella
:)
Please remove the name of your organization from your posting. I have mentioned this many times in your briefing.
Please use one of the test performed in the automated machine to explain the principle of the test and this test is used clinically. Your posting is more related to LMQA. You are also required to know the principle of the test performed and not just describing what you have done. Please have another posting by next Wed.
Hi Stella!
Since your lab depends on the analyzers, how many times do you need to do the control? And do you use the same level of control everytime?
Vanessa Chua!
0702099C
Grp 6
Hi stella.
do you need do quality control and calibration everytime you change the modular and catridge chemistry?
and how often did u do QC?
eg. 3 times per day
Nurul Nyzah
Hi Nyza!
Calibration is required when there is a change in a reagent lot, or when QC results are unacceptable or even after major maintenance and service.
It is a must to do quality control twice a day.
but for DxC, it is a must to do calibration followed by QC everyday :)
stella
0701059H
Hi Vanessa!
we need to do control two times each day.
and by the way can u rephrase your questin about the level of control? i don't quite get you. hehe. THANKS!
stella
0701059H
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