HI EVERYBODY!
stella here to post another entry!
I'm going to talk about Roche Cobas e601 Analyzer.
As i'm attached to a lab with mostly automated machines, therefore most of the tests are run on analyzers.
Roche Cobas e601 analyzer run different kinds of tests such as cardiac markers (CKMB, myoglobin, NTproBNP, Troponin T), tumour markers (AFP, CEA, CA125, CA15-3, free PSA, Total PSA) and bone markers (beta cross laps and osteocalcin)
An example of a test that is run on the Cobas e601 analyzer are cardiac markers such as Troponin T.
The purpose of performing the test is as follow:
Troponin T is useful and important in the diagnosis of myocardial damage such as myocardial infarction,
Principles of the tests : Immunoassay with Electrochemiluminescence technology
During the assay, the sample containing Troponin T is incubated with two monoclonal antibodies specific to the Troponin T. Examples of the antibodies against Troponin T are biotinylated monoclonalTroponin T-specific Antibody and a monoclonal troponin T-specific antibody labeled with a ruthenium complex. Thus, a sandwich complex is formed. Afterwhich, microparticles coated with streptavidin are added and the complex will be bound to to the solid phase through the interaction of biotin and streptavidin. The entire complex are drawn onto the electrode magnetically, with unbound components being washed away.
Electrochemiluminscent is induced by the application of a defined voltage. The photomultiplier measures the emission of light produced.
Reference interval for Troponin T:
<0.03ug/L
Troponin T can be futher divided into skeletal Troponin T and cardiac Troponin T.
Cardiac troponin T is a specific cardiac marker used for the diagnosis of myocardial damage.
It has unique cardiac specificity as it has different amino acid sequenes which are different from that of skeletal troponin T.
The presence of cardiac Troponin T in the bloodstream indicates there is myocardial damage.
In a healthy individual, no levels of troponin T can be detected in the bloodstream.
troponin T is a better cardiac marker due to its ability to stay in the bloodstream up to 2 weeks.
It rises from 4 to 6 hours, peaks at 12 to 24 hours and persist in the blood for 2 weeks.
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7 comments:
Hi erm. Why the T- Troponin need to be incubated with 2 antibodies that are coated with 2 different complex? And the solid phase is it something like microtiter plate?
Jennifer.
hi stella!
can i ask whats the function of streptavidin?
and what is ruthenium complex?
what is the advantages and disadvantages of using this method to detect troponin T ?
by the way, happy birthday! (:
LIM JIA HUI JOEY
tgo1 group2 0703605F
hi!
what are the other tests available for cobas?
and which tests cannot be run in this analyzer?
thanks:),
wendy.
hi joey! streptavidin is used to bind to biotin as it has great affinity to it. it has to be bind to e biotin as the monoclonal antibody is biotinylated.
and if im not mistaken, ruthenium complexes act as electrochemiluminescent in this method.
so far this method is good cos the whole assay takes about 18 minutes only. which is considered fast. and this assay is specific.
I'LL find out for you more on the advantages and disadvantages okay!
hopefully i answer ur ques! :)
stella
0701059H
hi jennifer!
it is incubated with 2 antibodies to form a sandwich complex. and the complexes serve different purposes such as to enhance e binding affinity to the electrochemiluminescent and paramagnetic particles.
and yes the solid phase is something like microtiter plate.
stella
0701059H
hi wendy!
so far the tests that i mentioned are all the tests that cobas can perform.
other tests such as cyclosporine, everolimus, valproic acid, carbamazepine etc are some examples of tests that cannot be run on cobas.
stella
0701059H
hi!
can you explain how advia 1800 works? it works on the principle of photometry, that i know. i actually wanted to know reagrding the biochemistry aspect of this machine.
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