TDX analyzer

hello everybody!
can u believe it!
its going to be the 13th week of our SIP!
yay yay!

alright lets get things started.
this week i'm allocated to one of the analyzers being used in the lab called TDX analyzer.

This TDX analyzer perform tests for therapeutic drugs such as Cyclosporine, Everolimus and Methotrexate .
The therapeutic drugs mentioned are examples of immunosuppressive drugs.

The purpose of performing tests on the TDX analyzer is to quantitatively measure the amount of drug administered in the body to monitor the levels of these drugs to ensure therapeutic dosage and to minimise the levels of toxic side-effects,

im going to talk about one of the tests performed which is Cyclosporine test.
Cyclosporine is effective in fighting against tissue rejection after an organ transplantation.

The Cyclosporine Monoclonal Whole Blood assay uses FPIA (FLuorescence Polarization Immunoassay) Technology as the principle of the test.

This is when there will be a competitive protein binding technology that utilizes the specificity of immunoassay with a fluorescent tracer in order to differentiate the bound from the free analyte. The analyte, in this case, is a drug/drug derivative. During the process, the analyte is labelled. A specific Antibody against the analyte will then be prepared. The assay will cause the excitation of fluorescein tracer with polarized light and the level of fluoresent polarization of tracer is measured. The concentration of analyte is inversely proportional to the polarized light,

Methods for The Cyclosporine Monoclonal Whole Blood assay :
1. Microcentrifuge tubes are labelled accordingly for each of the patient's sample
2. The sample is mixed with gentle inversion and 150ul of it is pipetted out into the corresponding microcentrifuge tubes.
3. 300ul of Whole Blood Precipitation Reagent/ Probe Wash is pipetted accurately into each microcentrifuge tube.
4. Each tube is capped securely to avoid any spillage and vortexed for 10 seconds to make sure thorough mixing.
5. The specimens are centrifuged 10800rpm for 5 minutes to obtain a clear supernatant and a hard compact pellet of denatured proteins.
6. the supernatant is decant completely into the corresponding sample well of a sample cartridge (supernatant's minimum volume required is 150ul)
7. The Reagent Pack is mixed with gentle inversion. The formation of bubbles is avoided.
8. The reagent Pack is placed on the reagent platform.
9. The sample carousel is placed onto the analyzer.
10. Close the cover of the analyzer and press RUN.

Additional info must be noted that Cyclosporine must undergo a pretreatment step prior executing the methods above to extract the drug from the blood sample.

:)

Stella
0701059H